%0 Journal Article
%T Determination of Tetramethylpyrazine in Animal Serum and Cerebrospinal Fluid b y High Performance Liquid Chromatography<br>反相高效液相色谱法测定实验动物血液和脑组织中川芎嗪
%A DING Ming-yu
%A LUO Shi-zhong
%A LIU Hai
%A CHEN Pei-rong
%A LIU De-lin
%A <br>丁明玉
%A 罗施中
%A 刘海
%A 陈培榕
%A 刘德麟
%J 色谱
%D 2000
%I 
%X A reversed-phase high performance liquid chromatographic (RP-HPLC) method for the determination of the tetramethylpyrazine(TMP) in Chuanxiong extract, the animal(mouse) serum and cerebrospinal fluid has been developed. The TMP was separated on an ODS column Zorbax SB-C18(4.6 mm i.d. x 250 mm, 5 microns) at room temperature and detected by using UV detector at 270 nm. The mobile phase was methanol-water (50:50, V/V) containing 0.2 mmol/L of NH4H2PO4 flowing at a rate of 0.8 mL/min and 20 microL samples were injected. The detection limit of TMP was 1 mg/L and the calibration curve is linear between 5 and 500 mg/L with a correlation coefficient (r) of 0.999. The recovery of TMP ranged 98%-103%. The extract of Chuanxiong and pretreated serum and cerebrospinal fluid sample are stable for a week at room temperature.
%K high performance liquid chromatography
%K tetramethylpyrazine
%K Chuanxiong
%K cerebrospinal fluid
%K serum<br>川芎嗪
%K 川芎
%K 脑组织
%K 血液
%K 动物实验
%K HPLC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=18AC600E35645725&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=D997634CFE9B6321&eid=B6DA1AC076E37400&journal_id=1000-8713&journal_name=色谱&referenced_num=6&reference_num=4