%0 Journal Article
%T Simultaneous determination of pantothenic acid and D-panthenol in cosmetics by high performance liquid chromatography<br>高效液相色谱法测定化妆品中的泛酸及D-泛醇
%A MAO Xiqin
%A HU Xia
%A PAN Wei
%A <br>毛希琴
%A 胡侠
%A 潘炜
%J 色谱
%D 2010
%I 
%X A high performance liquid chromatographic method (HPLC) and sample pretreatment method were developed for the simultaneous determination of pantothenic acid (vitamin B5) and D-panthenol (provitamin B5) in cosmetics with different matrices (including of creams, lotions, aqueous cosmetics, oily cosmetics, wax-based cosmetics, nail polish etc). A liquid-liquid extraction system composed of water and water-immiscible solvent was used to preliminarily separate the target components from other oil-soluble components and surfactants in cosmetics, then macromolecular water-soluble matrices in cosmetics were removed by coprecipitation with potassium ferrocyanide-zinc acetate precipitating agent, and then under acid condition, pantothenic acid and D-panthenol were enriched on a C18 solid-phase extraction sorbent. After the removal of other water-soluble impurities, target components were eluted by 40% methanol and then separated and quantitatively analyzed by high performance liquid chromatography with external standard method. Good linear relationship was achieved in the concentration range of 0.1~10 μg/g for pantothenic acid and D-panthenol. The linear correlation coefficients were separately 0.9989 and 0.9996. The average recoveries of the target components in cosmetics were more than 90%. Limit of detection of the method was 30 μg/g and the limit of quantification was 100 μg/g. This method can be used to simultaneously determine pantothenic acid and D-panthenol in cosmetics. The results are accurate and reliable.
%K high performance liquid chromatography (HPLC)
%K pantothenic acid
%K D-panthenol
%K cosmetic<br>高效液相色谱法
%K 泛酸
%K D-泛醇
%K 化妆品
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=476A83FD8A1A6D008C053693EF51A4AA&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=708DD6B15D2464E8&sid=29036368246435B2&eid=63025EFC6CC49145&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=0