%0 Journal Article
%T Analysis of Products of Thymine Irradiated by Heavy Ion Beam with Capillary Gas Chromatography<br>重离子辐照胸腺嘧啶产物的毛细管气相色谱分析
%A Cai Xichen
%A Wei Zengquan
%A Li Wenjian
%A Liang Jianping
%A Li Qiang
%A <br>蔡喜臣
%A 卫增泉
%A 李文建
%A 梁剑平
%A 李强
%J 色谱
%D 1999
%I 
%X In this study some chromatographic methods with capillary column, such as GC, GC-MS, GC-FTIR, were used to analyze the products of thymine irradiated by 18O8+ ion beam in N2O saturated aqueous solution. Because of the complexity and small amounts of the products after irradiation by heavy ion beams, it is very difficult to separate them, while with the methods of capillary gas chromatography this problem can be overcome. From the results of GC-MS analysis we can determine the molecular weight of the product, and from the results of GC-FTIR analysis we can get structure information of the product. By this way the products such as 5,6-dihydrothymine, 5-hydroxyl-5-methylhydantoin, 5-hydroxyl-6-hydrothymine, 5-hydro-6-hydroxylthymine, 5-hydroxymethyluracil, trans-thymine glycol, cis-thymine glycol and dimers were determined. Though these products are the same as those from thymine irradiated by gamma ray in N2O saturated aqueous solution, the mechanism of heavy ion beam interact ion with thymine aqueous solution is different from that of gamma ray. The main products from thymine irradiated by 18O8+ ion beam in N2O saturated aqueous solution are formed by hydroxyl additions between 5-6 band of thymine, while those by gamma ray are dimers of thymine.
%K capillary gas chromatography
%K irradiated by heavy ion beam
%K thymine<br>毛细管气相色谱
%K 重离子辐照
%K 胸腺嘧啶
%K 产物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B86285C900C1768ACFC9&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=94C357A881DFC066&sid=5335AD3CFE6E14EA&eid=F27A401E323B6FAD&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=1