%0 Journal Article
%T Identification of the authentic quality of Longdanxiegan pill by systematic quantified fingerprint method based on three wavelength fusion chromatogram<br>基于三波长融合谱的系统指纹定量法鉴定龙胆泻肝丸的真实质量
%A SUN Guoxiang
%A ZHANG Jingxian
%A <br>孙国祥
%A 张静娴
%J 色谱
%D 2009
%I 
%X The three wavelength fusion high performance liquid chromatographic fingerprints (TWFFP) of Longdanxiegan pill (LDXGP) was established to identify the quality of LDXGP by the systematic quantified fingerprint method. The chromatographic fingerprints (CFPs) of the 12 batches of LDXGP were determined by reversed-phase high performance liquid chromatography. The technique of multi-wavelength fusion fingerprint was applied during processing the fingerprints. The TWFFPs containing 63 co-possessing peaks were obtained when choosing baicalin peak as the referential peak. The 12 batches of LDXGP were identified with hierarchical clustering analysis by using macro qualitative similarity (Sm) as the variable. According to the results of classification, the referential fingerprint (RFP) was synthesized from 10 batches of LDXGP. Taking the RFP for the qualified model, all the 12 batches of LDXGP were evaluated by the systematic quantified fingerprint method. Among the 12 batches of LDXGP, 9 batches were completely qualified, the contents of 1 batch were obviously higher while the chemical constituents quantity and distributed proportion in 2 batches were not qualified. The systematic quantified fingerprint method based on the technique of multi-wavelength fusion fingerprint can effectively identify the authentic quality of traditional Chinese medicine.
%K high performance liquid chromatography (HPLC)
%K systematic quantified fingerprint method
%K three wavelength fusion fingerprint
%K macro qualitative similarity
%K macro quantitative similarity
%K Longdanxiegan pill (LDXGP)<br>高效液相色谱法
%K 系统指纹定量法
%K 三波长融合指纹图谱
%K 宏定性相似度
%K 宏定量相似度
%K 龙胆泻肝丸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=E2C41F15FCFB9E368288AAD1EB954609&yid=DE12191FBD62783C&vid=DB817633AA4F79B9&iid=38B194292C032A66&sid=3C6F5C97A07587AE&eid=3224764AEAFCF8C2&journal_id=1000-8713&journal_name=色谱&referenced_num=7&reference_num=11