%0 Journal Article
%T Determination of Activity of Bone Alkaline Phosphatase in Whole Serum by High Performance Liquid Chromatography<br>高效液相色谱法测定全血中骨碱性磷酸酶的活力
%A ZHANG Yong-mei
%A BAI Xiu-zhen
%A CHEN Zhan-wen
%A ZHANG Chun-xian
%A <br>张咏梅
%A 白秀珍
%A 陈占文
%A 张春先
%J 色谱
%D 2000
%I 
%X An analytical method has been established for the determination of bone alkaline phosphatase (BALP) activity using high performance liquid chromatography(HPLC). The stainless steel column was 22 cm x 4.6 mm i.d. packed with totally porous, spherical silica particles. A solution of methanol and 0.01 mol/L CH3COOH(30/70, V/V) adjusted to pH 4.8 was employed as the mobile phase. The flow rate was 0.8 mL/min. Chromatography was performed with ultraviolet detector at 275 nm. Linear calibration curve for BALP was measured within the range of 8-200 mg/L with correlation coefficient of 0.996. The lowest detection limit was 3 ng. The HPLC method described is simple, rapid and reliable and suitable for clinical monitoring.
%K high
%K performance
%K liquid
%K chromatography
%K bone
%K alkaline phosphatase
%K whole
%K blood<br>高效液相色谱法
%K 骨碱性磷酸酶
%K 全血
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=059C16A1684964B05232A94524D03F9D&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=38B194292C032A66&sid=6CCE24D86D03D083&eid=FBCA02DBD05BD4EA&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=3