%0 Journal Article
%T Determination of Itaconic Acid in Culture Broth by High Performance Liquid Chromatography<br>高效液相色谱法测定发酵醪液中的衣康酸
%A Lu Zhibin
%A Wang Qian
%A Lei Zhifang
%A Zhang Hongxun
%A <br>鲁治滨
%A 王茜
%A 雷志芳
%A 张洪勋
%J 色谱
%D 1999
%I 
%X An HPLC method for the determination of itaconic acid and other components in the culture broth of Aspergillus terreus was developed. The operating conditions were Aminex HPX-87H column (300 mm x 7.8 mm) at 30 degrees C, mobile phase of sulfuric acid (4 mmol/L) and refractive index detector at 30 degrees C. The linear relationship of calibration curve was good in the range of 0-50 g/L for itaconic acid (r = 0.9997). The detection limit was 5.7 micrograms, and the relative standard deviation was 1.37% (n = 7). Other components in the culture broth were SO(4)2-, sucrose, glucose and fructose. The method is simple, sensitive, rapid and accurate.
%K high performance liquid chromatography
%K culture broth
%K Itaconic acid<br>发酵醪液
%K 衣康酸
%K HPLC
%K 甲叉丁二酸
%K 测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B862E9E45531D4FB384F&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=E158A972A605785F&sid=AFB2C35009D5D191&eid=5A735990D5DE8BF4&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=0