%0 Journal Article
%T Determination of alliin and its related substances in garlic using pre-column derivatization and reversed-phase high performance liquid chromatography<br>AQC柱前衍生化RP-HPLC法测定蒜氨酸及其有关物质的含量
%A YUAN Yaozuo
%A HANG Taijun
%A JI Yu
%A ZHANG Zhengxing
%A <br>袁耀佐
%A 杭太俊
%A 纪宇
%A 张正行
%J 色谱
%D 2008
%I 
%X A reversed-phase high performance liquid chromatographic method with pre-column derivatization for the determination of alliin and its related substances, which are the precursors of garlic's active components, was established. Alliin was derivatized with 6-aminoquinolyl-N-hydroxysuccinimicly carbamate (AQC). The reaction of derivatization was very fast and the derivative was stable. The analysis was carried out on a Kromasil C18 column (250 mm x 4.6 mm, 5 microm) with a gradientelution and detection at 248 nm. The mobile ph ase consisted of 0.1% acetamide (0.03% acetic acid) (A) and the mixture of water and acetonitrile (40: 60, v/v) (B), and the flow rate was set at 1.0 mL/min. The linear calibration was found for alliin within the range of 1.171 9 -1 500 microg/mL (r = 0.999 8). The inter-day and intra-day precision were good with relative standard deviation (RSD) less than 1.8% (n = 5). The recovery was 99.1% with the RSD of 1.9%. The limit of detection was 0.15 microg/mL. The method established is accurate, simple and rapid and suitable for the determination of alliin and related substances.
%K 反相高效液相色谱法
%K 柱前衍生化
%K 蒜氨酸及其有关物质
%K 大蒜
%K 柱前衍生化
%K 反相高效液相
%K 色谱法测定
%K 大蒜
%K 蒜氨酸
%K 有关物质
%K liquid
%K chromatography
%K high
%K performance
%K derivatization
%K garlic
%K related
%K substances
%K 快速
%K 方法
%K 浓度
%K 最小
%K 添加回收率
%K 相对标准偏差
%K 精密度
%K 线性关系
%K 范围
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=2A35482C0EA946BE021B0C2E7171E446&yid=67289AFF6305E306&vid=96C778EE049EE47D&iid=0B39A22176CE99FB&sid=0C191C6ECF79047F&eid=E2E0FBFE4D7EFB94&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=12