%0 Journal Article
%T Isolation and Purification of Solanesol from Potato Leaves by High-Speed Counter-Current Chromatography and Identification by Atmospheric Pressure Chemical Ionization Mass Spectrometry<br>土豆叶中茄尼醇的高速逆流色谱法分离纯化及质谱解析
%A HU Jiangyong
%A LIANG Yong
%A XIE Ya
%A HUANG Zhaofeng
%A ZHONG Hanzuo
%A <br>胡江涌
%A 梁勇
%A 谢亚
%A 黄肇锋
%A 钟汉佐
%J 色谱
%D 2007
%I 
%X Preparative high-speed counter-current chromatography (HSCCC) was used for the isolation and purification of solanesol from potato leaves. Experimental conditions of the extraction of solanesol from potato leaves have been optimized. An ultrafine extraction method was applied in this study. The efficiency using an ultrafine extraction was found to be improved in the investigation, the yields of solanesol by different extraction methods were 0.083% by ultrafine extraction and 0.050% by ultrasonic extraction. Using n-hexane-methanol (10:7, v/v) as the two-phase solvent system, preparative HSCCC was successfully performed with the yield of 5 mg solanesol at 98.7% of purity from 60 mg of crude extract in the one-step separation. The mobile phase was the lower phase and operated at a flow rate of 1.5 mL/min, while the apparatus rotated at 800 r/min. The solanesol was identified by the atmospheric pressure chemical ionization mass spectrometry (APCI-MS). The ionization and cleavage mechanisms of solanesol in APCI-MS and APCI-MS/MS are discussed.
%K high-speed counter-current chromatography(HSCCC)
%K atmospheric pressure chemical ionization mass spectrometry(APCI-MS)
%K solanesol
%K potato leaves
%K preparative isolation<br>高速逆流色谱
%K 大气压化学电离质谱
%K 茄尼醇
%K 土豆叶
%K 制备分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A04536C928D3B546&yid=A732AF04DDA03BB3&vid=C5154311167311FE&iid=E158A972A605785F&sid=652E7E360EBE3082&eid=20ADD38F841C6A4B&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=9