%0 Journal Article
%T Synthesis of 1-[2-(p-Toluenesulfonate)Ethyl]-2-Phenylimidazole [4,5-f] 9,10-Phenanthrene and Its Application for Analysis of Long-Chain Fatty Acids<br>荧光衍生试剂1-［2-(对甲苯磺酸酯)乙基］-2-苯基咪唑［4,5-f］9,10-菲的合成及其在长链脂肪酸分析中的应用
%A ZHAO Xian'en
%A SUO Yourui
%A DING Chenxu
%A ZHU Fang
%A SUN Jing
%A ZHAO Wenchen
%A SUN Xuejun
%A YOU Jinmao
%A <br>赵先恩
%A 索有瑞
%A 丁晨旭
%A 朱芳
%A 孙菁
%A 赵文臣
%A 孙学军
%A 尤进茂
%J 色谱
%D 2006
%I 
%X A simple and sensitive method for the determination of long-chain free fatty acids (FFAs) using 1-2-(p-toluenesulfonate) ethyl]-2-phenylimidazole 4, 5-f] 9, 10-phenanthrene (TSEPIP) as pre-column derivatization reagent by reversed-phase high performance liquid chromatography with fluorescence detection and mass spectrometric identification was developed. Eleven long-chain (C20-C30) FFA derivatives were separated on Eclipse XDB-C8 column with a good baseline resolution. Studies on derivatization conditions indicate that FFA reacts rapidly and smoothly with TSEPIP in the presence of K2CO3, catalyst at 90 degrees C in N, N-dimethylformamide solvent to give the corresponding sensitively fluorescent derivatives with maximal yields close to 100% with a 5-fold molar reagent excess. The identification of 11 FFA derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization source in positive-ion mode. The contents of 11 FFAs in soil and three bryophytes (Homomallium connexum (Card.) Broth., Actinothuidium hookeri, Neckera pennata) were determined. The results indicate that the bryophyte plants enrich an abundance of FFAs from soil. The excitation and emission wavelengths of fluorescence detection were set at 260 nm and 380 nm, respectively. Linear correlation coefficients for the most FFA derivatives were higher than 0.9996, and the detection limits (at signal-to-noise of 3:1) were 26.19-76.67 fmol. The method is sensitive and reproducible for the determination of long-chain FFAs in real samples.
%K high performance liquid chromatography-mass spectrometry(HPLC-MS)
%K fluorescence derivatization reagent
%K fluorescence detection
%K pre-column derivatization
%K long-chain fatty acids<br>高效液相色谱-质谱法
%K 荧光衍生试剂
%K 荧光检测
%K 柱前衍生
%K 长链脂肪酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=4FDBED735D607D8A&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=522844664D9E629A&eid=FCACFF68346F8D4F&journal_id=1000-8713&journal_name=色谱&referenced_num=5&reference_num=9