%0 Journal Article
%T Simultaneous Determination of Five Groups of Components in Qingkailing Injection by High Performance Liquid Chromatography with Photo Diode Array Detector and Evaporative Light Scattering Detector<br>应用高效液相色谱-二极管阵列检测器-蒸发光散射检测器联用技术同时测定清开灵注射液中的五类有效成分
%A YAN Shikai
%A XIN Wenfeng
%A LUO Guoan
%A WANG Yiming
%A CHENG Yiyu
%A <br>严诗楷
%A 辛文峰
%A 罗国安
%A 王义明
%A 程翼宇
%J 色谱
%D 2005
%I 
%X A method was established for the simultaneous quantification of nine components of five different structural types in Qingkailing injection. High performance liquid chromatography coupled with a photo diode array detector and an evaporative light scattering detector (HPLC-DAD-ELSD) was employed in the determination. Four monitoring wavelengths of 240, 254, 280 and 330 nm were set to determine nucleosides (uridine and adenosine), iridoid glucoside (geniposide), flavone glycoside (baicalin) and organic acids (chlorogenic acid and caffeic acid) respectively, and a combined evaporative light scattering detector was used to detect three steroid compounds (cholic acid, ursodesoxycholic acid and hyodeoxycholic acid). The proposed method permitted the simultaneous separation and determination of five groups of compounds in Qingkailing injection, and acceptable validation results of the precision, repeatability, stability and accuracy tests were achieved. The method was applied to the analysis of 19 Qingkailing injection samples from three different plants, and the results indicated that the method could be used as a convenient and reliable method in the multi-component determination and quality (control) of traditional Chinese medicines.
%K high performance liquid chromatography
%K photo diode array detector
%K evaporative light scattering detector
%K active constituents
%K Qingkailing injection<br>高效液相色谱法
%K 二极管阵列检测器
%K 蒸发光散射检测器
%K 有效成分
%K 清开灵注射液
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=86E425D983AC9567&yid=2DD7160C83D0ACED&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=BE5DBC360CD4FFB9&eid=8C8D895E58E44DBB&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=12