%0 Journal Article
%T Determination of four carcinogens in mainstream cigarette smoke by liquid chromatography-tandem mass spectrometry<br>液相色谱-串联质谱法测定卷烟主流烟气中的四种致癌物
%A HOU Hongwei
%A XIONG Wei
%A TANG Gangling
%A HU Qingyuan
%A <br>侯宏卫
%A 熊巍
%A 唐纲岭
%A 胡清源
%J 色谱
%D 2010
%I 
%X N-nitrosonornicotine (NNN), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosoanatabine (NAT) and N-nitrosoanabasine (NAB) are the most abundant carcinogens identified in tobacco and tobacco smoke. The accurate quantifications of NNN, NNK, NAT and NAB are necessary to evaluate its impact on the public health. A liquid chromatography-electrospray tandem mass spectrometry (LC-ESI MS/MS) method was developed to simultaneously determine NNN, NNK, NAT and NAB in mainstream cigarette smoke. Mainstream smoke was collected in a Cambridge filter pad and then was extracted by 10 mL 100 mmol/L ammonium acetate after 100 μL of mixed deuterated internal standards was added. Then the extract was detected by using positive electrospray ionization on a tandem mass spectrometer in multiple reaction monitoring (MRM) mode. NNN, NNK, NAT and NAB were separated on a Zorbax Eclipse XDB-C18 column with the gradient elution using mobile phase A (0.1% acetic acid in water) and mobile phase B (0.1% acetic acid in methanol). The detection limits for NNN, NNK, NAT and NAB were 0.019, 0.002, 0.008 and 0.007 μg/L, respectively. The recoveries were varied from 84.9% to 104.5% for Chinese Virginia cigarettes and the relative standard deviations (n=8) ranged from 2.96% to 6.65%. This proposed approach, which provides a higher sensitivity and specificity, is suitable for the determination of NNN, NNK, NAT and NAB in mainstream cigarette smoke.
%K liquid chromatography-electrospray tandem mass spectrometry (LC-ESI MS/MS)
%K N-nitrosonornicotine
%K 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
%K N-nitrosoanatabine
%K N-nitrosoanabasine
%K mainstream cigarette smoke
%K Chinese Virginia cigarettes
%K blended cigarettes<br>液相色谱-串联质谱法
%K N-亚硝基降烟碱
%K 4-(亚硝基甲氨基)-1-(3-吡啶基)-1-丁酮
%K N-亚硝基新烟草碱
%K N-亚硝基假木贼碱
%K 主流烟气
%K 中国烤烟型卷烟
%K 混合型卷烟
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=FD8B281592055F705FD1E6B3D4B367B6&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=5D311CA918CA9A03&sid=9409F3EB075DCD5B&eid=3D8AB54CA690066A&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=18