%0 Journal Article
%T Determination of Simvastatin in Human Plasma by High Performance Liquid Chromatography<br>高效液相色谱法测定人血浆中的辛伐他汀
%A TAN Li
%A YANG Li-li
%A ZHANG Xin
%A YUAN Yi-sheng
%A LING Shu-sen
%A <br>谭力
%A 杨丽莉
%A 张昕
%A 袁倚盛
%A 凌树森
%J 色谱
%D 2000
%I 
%X Simvastatin is widely used in the treatment of hypercholestero-lemia. Recently, it was reported that it is also effective in reducing lethality in coronary heart disease. A simple and sensitive reversed-phase liquid chromatographic method has been developed and validated for the analysis of simvastatin in human plasma. Simvastatin and internal standard lovastatin in plasma were extracted with cyclohexane-dichloromethane (3.5:1, V/V), and then measured by HPLC using a Lichrospher C18 column as stationary phase and an acetonitrile-water (70:30, V/V) mixture as mobile phase. The flow rate was 1.2 mL/min. Simvastatin was quantified by UV at 237 nm. The calibration curve showed a good linearity in the mass concentration range of 0.25-50.0 micrograms/L. The regression equation is: Y = 0.0364 rho + 0.00583, r = 0.9998. Intra-day and inter-day coefficients of variation of assay for simvastatin in plasma < 7.94% and < 8.58%, respectively. The recoveries of simvastatin were > 93.3%. The method has been used to determine simvastatin in plasma from 12 healthy male volunteers.
%K high performance liquid chromatography
%K simvastatin
%K plasma concentration<br>高效液相色谱法
%K 辛伐他汀
%K 血药浓度
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=83DD5A5A94A289BBFDA502CA4B9419A1&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=38B194292C032A66&sid=E1D946F217E3B046&eid=D9AE183D3F5C3C75&journal_id=1000-8713&journal_name=色谱&referenced_num=3&reference_num=0