%0 Journal Article
%T Separation of Isomeric Compounds by High Speed Countercurrent Chromatography<br>高速逆流色谱分离同分异构体
%A CHEN Li
%A DENG Lijie
%A CHEN Ping
%A <br>陈理
%A 邓丽杰
%A 陈平
%J 色谱
%D 2006
%I 
%X High speed countercurrent chromatography (HSCCC) was successfully used for the separation and preparation of isomeric compounds. The influence of various two-phase solvent systems on the resolution of the natural catechin isomers of (-) -epigallocatechin gallate (EGCG) and (-) -gallocatechin gallate (GCG) from green tea polyphenols and the synthetic isomeric compounds of para-, ortho-, meta-bromoanilline was investigated. The results indicated that the catechin isomers of (-) -EGCG and (-) -GCG can be isolated through two runs of preparative HSCCC with two-phase solvent system composed of n-hexane-ethyl acetate-water (1: 10: 10 in volume ratio). The upper organic phase was used as stationary phase and the lower aqueous phase as mobile phase, while the mobile phase was operated at a flow rate of 3.5 mL/min, and the apparatus rotated at 800 r/min. High performance liquid chromatographic analysis of (-) -EGCG and (-) -GCG revealed that the purity was all over 98%. The isomeric compounds of para-, ortho-, meta-bromoaniline can also be effectively isolated through a single run of preparative HSCCC with the mobile phase flow rate of 3.0 mL/min and the apparatus rotation speed of 800 r/min by using tetrachloromethane-chloroform-methanol-water (7: 3: 7: 3 in volume ratio) as two-phase solvent system, while the lower phase was selected as stationary phase and the upper phase as mobile phase.
%K high speed countercurrent chromatography(HSCCC)
%K isomeric compounds
%K catechin
%K bromoaniline
%K separation<br>高速逆流色谱法
%K 同分异构体
%K 儿茶素
%K 溴代苯胺
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=F8510C7A8BA283B2&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=B31275AF3241DB2D&sid=18F040DBCB74FFF9&eid=03EE8EDD44A3D4BE&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=9