%0 Journal Article
%T Albumin-Drug Binding Study by Capillary Electrophoresis I. Quantitative Applicability Examination of Liquid Pre-Column<br>人血清白蛋白与手性药物相巨作用的毛细管电泳研究Ⅰ.液相预柱毛细管电泳技术定量可靠性的考察
%A Ding Yongsheng
%A Lin Bingcheng
%A <br>丁永生
%A 林炳承
%J 色谱
%D 1999
%I 
%X To measure the free concentration of verapamil (a basic drug) enantiomers in the binding system of human serum albumin(HSA), a capillary electrophoretic method, liquid precolumn(LCP), was established, and the method was examined systematically. In physiological pH condition (pH 7.4, ionic strength 0.17), HSA migrates in the opposite direction of verapamil. This electrophoretic property basically supposed the probability of preventing HSA from entering the capillary whereas a positive electric field was used. Finally, the drug enantiomers were separated by the chiral selector (45 mmol/L trimethyl-beta-cyclodextrin, pH 2.5 phosphate buffer) and the free concentration of each optical isomer in the binding system was measured. Seven samples were examined and their relative standard deviations(RSD) and the relative errors (RE) of unbound drug were 2.1%-5.02% and 1.4%-5.8%, respectively.
%K capillary electrophoresis
%K liquid pre-column
%K binding
%K human serum albumin
%K drug<br>毛细管电泳
%K 液相预柱
%K 相互作用
%K 人血清白蛋白
%K 药物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=522BF4BCABDB614FC005D8781633DD6F&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=03A030BB0C519C60&eid=205BE674D84A456D&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=0