%0 Journal Article
%T Simultaneous Determination of 18 Amino Acids by Reversed-Phase High Performance Liquid Chromatography with Precolumn Phenylisothiocyanate Derivatization<br>异硫氰酸苯酯柱前衍生化反相高效液相法同时测定18种氨基酸
%A YANG Jing
%A SUN Li guang
%A BAI Xiu zhen
%A ZHOU Hai tao
%A <br>杨菁
%A 孙黎光
%A 白秀珍
%A 周海涛
%J 色谱
%D 2002
%I 
%X A reversed-phase high performance liquid chromatographic method for simultaneous determination of 18 amino acids is described. The sample was mixed with norleucine as the internal standard, then derivatized with phenylisothiocyanate (PITC) and analyzed on a Kromasil C18 column at 38 degrees C, using gradient elution with detection at 254 nm. The correlation coefficients between the ratios of peak area of amino acid to that of the internal standard and the amino acid concentrations were above 0.99, except for cysteine(Cys)(0.962). The recoveries of amino acids added were 96.0%-102.4%. Detection limit of leucine was 0.5 mg/L. The method was applied to analyze free amino acids in deproteinized calf blood injection and good results were obtained.
%K high performance liquid chromatography
%K precolumn derivatization
%K phenylisothiocyanate
%K norleucine
%K amino acid<br>同时测定
%K 高效液相色谱法
%K 柱前衍生化
%K 异硫氰酸苯酯
%K 正亮氨酸
%K 氨基酸
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=AC23CD34DABE08C8&yid=C3ACC247184A22C1&vid=A04140E723CB732E&iid=E158A972A605785F&sid=F7BB24011DC0D223&eid=23410D0BDB501DF5&journal_id=1000-8713&journal_name=色谱&referenced_num=28&reference_num=8