%0 Journal Article
%T Study of Diphacinone in Biological Samples byHigh Performance Liquid Chromatography/Diode Array Detector<br>高效液相色谱法分析生物样品中的敌鼠
%A YANG Shi yun
%A PAN Guan min
%A MENG Guang fan
%A ZHANG Da ming
%A <br>杨士云
%A 潘冠民
%A 孟广范
%A 张大明
%J 色谱
%D 2001
%I 
%X An analytical approach has been developed for high performance liquid chromatographic determination of diphacinone extracted from liver, blood, urine and kidney of rabbit by solid phase extraction (SPE) cartridges (using SAX, CN or SILICA GEL) with coumarin as the internal standard. Diphacinone was separated by reversed-phase gradient chromatography with DAD detection at 286 nm. The Analytical column was Hypersil BDS C18(150 mm x 4.6 mm i.d., 5 microns) and the guard column was Phenomenex ODS(4 mm x 3.0 mm i.d.). The mobile phase was a gradient mixture of aqueous solution (A) and methanol solution (B) both containing 0.5% ion pair A. There was a good linear relationship between the concentration of diphacinone and the ratio of peak areas of diphacinone and coumarin (internal standard) (r = 0.9999). The linear range was 1 mg/L-100 mg/L, and the lower detection limit was 5 ng (S/N = 3). The average recoveries of diphacinone in urine, blood and liver were 88.4% (n = 3, RSD = 1.25%, SPE by CN column), 82.2% (n = 3, RSD = 1.67%, SPE by SAX column), 91.0% (n = 3, RSD = 2.77%, SPE by SILICA GEL column), respectively.
%K high performance liquid chromatography/diode array detector
%K diphacinone
%K solid phase extraction<br>高效液相色谱法
%K 二极管阵列检测器
%K 敌鼠
%K 固相萃取
%K 生物样品
%K 抗凝血鼠药
%K 药物分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=B9E1235134A0FF24&yid=14E7EF987E4155E6&vid=2A8D03AD8076A2E3&iid=38B194292C032A66&sid=E2E0FBFE4D7EFB94&eid=002786F01A86D891&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=5