%0 Journal Article
%T Determination of monoethylglycinexylidide concentration in serum using solid phase extraction and capillary gas chromatography-mass spectrometry<br>固相萃取-气相色谱-质谱法测定利多卡因代谢物单乙基甘氨酰二甲苯胺的血药浓度
%A WANG Zhenxia
%A ZHANG Ruiming
%A CAO Wei
%A OUYANG Xiaohui
%A ZHAO Haiping
%A DONG Peide
%A MENG Xingkai
%A <br>王震侠
%A 张瑞明
%A 曹伟
%A 欧阳晓晖
%A 赵海平
%A 董培德
%A 孟兴凯
%J 色谱
%D 2008
%I 
%X A novel method for the determination of monoethylglycinexylidide (MEGX) (lidocaine metabolin) in serum using solid phase extraction (SPE) and capillary gas chromatography-mass spectrometry (GC-MS) was established. The serum sample was extracted with a CN-SPE column. An HP-5MS capillary column (15 m x 0.25 mm x 0.1 microm) was used. The initial temperature of the column was set at 100 degrees C, held for 1 min, then raised to 200 degrees C at 40 degrees C/min, and held at 200 degrees C for 0.5 min. The sample size was 2 microL, and the split ratio was set at 1 : 1. The carrier gas was high purity helium with a flow rate of 1.0 mL/min. The monitoring ions for the determination were m/z 58 for MEGX and m/z 86 for procaine (internal standard). The calibration curve of MEGX had good linear relationship in the range of 1.562 - 25 ng/mL ( r = 0.998 1). The limit of detection was 0.5 ng/mL. The extraction recovery ranged from 80.1% to 85.7%. The method advanced the quantitative analysis of MEGX in serum by combining rapid and efficient SPE with specific and sensitive quantitation by GC-MS.
%K gas chromatography-mass spectrometry (GC-MS)&prev_q=gas chromatography-mass spectrometry (GC-MS)')">gas chromatography-mass spectrometry (GC-MS)" target="_blank">gas chromatography-mass spectrometry (GC-MS)')">gas chromatography-mass spectrometry (GC-MS)
%K solid phase extraction (SPE)
%K monoethylglycinexylidide
%K lidocaine
%K metabolin
%K serum&prev_q=')">serum" target="_blank">')">serum<br>气相色谱-质谱联用法
%K 固相萃取
%K 单乙基甘氨酰二甲苯胺
%K 利多卡因
%K 代谢物
%K 血清
%K 固相萃取
%K 气相色谱
%K 质谱法
%K 测定
%K 利多卡因
%K 代谢物
%K 单乙基甘氨酰二甲苯胺
%K 血药浓度
%K spectrometry
%K capillary
%K solid
%K phase
%K ex
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=79F0F18583013AED448EA737F191C796&yid=67289AFF6305E306&vid=96C778EE049EE47D&iid=38B194292C032A66&sid=85002451B65CE0D1&eid=C2F76551C0111538&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=10