%0 Journal Article
%T Preparation of gentiopicroside from the extract of Swertia mussotii Franch by preparative high performance liquid chromatography<br>制备型高效液相色谱法分离纯化川西獐牙菜提取物中的龙胆苦苷
%A LEI Yujia
%A GAO Mingzhe
%A WANG Li
%A XIAO Hongbin
%A <br>雷宇佳
%A 高明哲
%A 王莉
%A 肖红斌
%J 色谱
%D 2010
%I 
%X A new method for isolation of gentiopicroside (GPS) from Swertia mussotii Franch with reversed phase preparative high performance liquid chromatography (Pre-HPLC) is described. After 95% ethanol extract was obtained with solvent extraction and macroporous resin separation, a fraction containing GPS was acquired and subjected to Pre-HPLC for isolation of target component. The optimum operation parameters were selected as follows: a C18 column (200 mm×50 mm, 5 μm), methanol-0.1% acetic water (30:70, v/v) as the mobile phase at flow rate of 75 mL/min, the detection wavelength at 254 nm, and the injection volume of 500 μL at the mass concentration of 225 g/L. The purity of the product was detected by HPLC method. The result showed that the purity of product was above 99%. The effective and rapid method has been successfully applied to the preparation of GPS from Swertia mussotii Franch.
%K preparative high performance liquid chromatography (Pre-HPLC)
%K gentiopicroside
%K Swertia mussotii Franch<br>制备型高效液相色谱法
%K 龙胆苦苷
%K 川西獐牙菜
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D215A697084D5DD18232838587B7D10C&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=9CF7A0430CBB2DFD&sid=F52FA2254444BE97&eid=46CB56AABC2765FF&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=13