%0 Journal Article
%T Determination of Tetracyclines Antibiotics by Reversed-Phase High Performance Liquid Chromatography<br>反相高效液相法测定四环素类抗生素
%A WANG Lei
%A ZHANG Xiao song
%A XU Zhi xiu
%A SHAO Xue guang
%A <br>王蕾
%A 张孝松
%A 徐智秀
%A 邵学广
%J 色谱
%D 2002
%I 
%X A method for the determination of 7 tetracycline antibiotics (TCs) by reversed-phase high performance liquid chromatography is described. TCs were successfully separated on a Diamonsil C18 column (250 mm x 4.6 mm i.d., 5 microns) using methanol-acetonitrile-0.01 mol/L oxalic acid (pH 2.0) (11:22:67, volume ratio) as mobile phase at a flow rate of 0.8 mL/min and detected at 267 nm within 22 min. Effects of the pH value of the mobile phase, concentration of the mobile phase buffer, elution composition and detection wavelength on the response and retention were studied. Tetracycline (TC) and oxytetracycline(OTC) in medicinal tablets were quantitated by standard added method. It has been proven that the method is fast, accurate and suitable for routine analysis.
%K reversed
%K phase high performance liquid chromatography
%K tetracycline antibiotics
%K UV
%K detection<br>反相高效液相色谱法
%K 测定
%K 四环素类抗生素
%K 紫外检测
%K 分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=0AEBA6BCF9EEE3AE&yid=C3ACC247184A22C1&vid=A04140E723CB732E&iid=CA4FD0336C81A37A&sid=2A3781E88AB1776F&eid=987EDA49D8A7A635&journal_id=1000-8713&journal_name=色谱&referenced_num=15&reference_num=8