%0 Journal Article
%T Isolation and Preparation of Sesquiterpenols from the Japanese Cedar, Cryptomeria japonica D. Don by Preparative Reversed-Phase High Performance Liquid Chromatography and Its Characterization<br>制备反相高效液相色谱法用于从杉材中分离制备倍半萜类化合物
%A CHEN Xiaohui
%A ZHANG Huifen
%A BI Kaishun
%A KIM Chul-Sa
%A HORIIKE Michio
%A <br>陈晓辉
%A 张晖芬
%A 毕开顺
%A 金哲史
%A 崛池 道郎
%J 色谱
%D 2005
%I 
%X A method for the isolation of sesquiterpenols from the Japanese Cedar, Cryptomeria japonica D. Don by preparative reversed-phase high performance liquid chromatography (RP-HPLC) was established. The preparation of the sesquiterpenols was carried out on a preparative liquid chromatograph with a YWC-Pack C18 column, and the mobile phase was isopropanol-methanol-hexane-water (50:35:10:5, v/v) at a flow rate of 5.0 mL/min. The two sesquiterpenols, (-)-cubebol and (+)-2,7(14),10-bisabolatrien-1-ol-4-one, were separated by liquid chromatography and identified by spectroscopic analyses (ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS)). The purities of the two sesquiterpenols were 98.7% and 99.1%, respectively.
%K preparative high performance liquid chromatography
%K chemical constituents of sesquiterpenols
%K Cryptomeria japonica D
%K Don<br>制备液相色谱
%K 倍半萜类化学成分
%K 杉材
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=FB3DF57FBA0DCC10&yid=2DD7160C83D0ACED&vid=EA389574707BDED3&iid=CA4FD0336C81A37A&sid=CD775AE9DDBD7B53&eid=117F81797AB182FC&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=12