%0 Journal Article %T Study on the Separation of Proteins in Human Serum by High Performance Capillary Zone Electrophoresis
高效毛细管区带电泳法分离人血清蛋白质的研究 %A LI Ke %A YUAN Yi-sheng %A ZHAO Fei-lang %A
李克 %A 袁倚盛 %A 赵飞浪 %J 色谱 %D 2000 %I %X A high performance capillary zone electrophoretic method has been developed for the separation of proteins in human serum. Sera were diluted with a buffer (0.05 mol/L boric acid, pH 8.80), and then introduced hydrodynamically into a 37 cm (32 cm to detector) x 50 microns i.d. fused-silica capillary, electrophoresed for 12 min using a boric buffer solution (0.10 mol/L, pH 9.35; containing 4 g/L of polyethylene glycol 8000), and detected at the wavelength of 200 nm. The method is simple, rapid and repeatable. %K high performance capillary zone electrophoresis %K protein %K serum
毛细管区带电泳 %K 蛋白质 %K 血清蛋白 %K 分离 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=9BA2AC08419A7FD8CE61F77F0663B090&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=0B39A22176CE99FB&sid=04445C1D2BDA24EE&eid=E2546871E5B846EF&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=6