%0 Journal Article
%T Capillary gas chromatographic analysis and mass spectrometric identification of modified DNA bases 5-methylcytosine and 8-hydroxyguanine]<br>DNA修饰碱基5-甲基胞嘧啶和8-羟基鸟嘌呤的毛细管气相色谱分析及质谱鉴定
%A Y Z Song
%A Q F Zhu
%A H Q Zhuang
%A L E Mo
%A <br>宋元宗
%A 祝其锋
%A 庄海旗
%A 莫丽儿
%J 色谱
%D 2000
%I 
%X The purpose of this paper was to establish a method for the determination of modified bases, 5-methylcytosine and 8-hydroxyguanine, in DNA by GC/FID. The experimental conditions were explored systematically for the quantitative analysis of these two modified bases, and the components were identified by GC/MS. The results showed that the variant components in DNA treated with Fenton's reaction can be derivatized and separated successfully. The relative molar reactive factors of 5-methylcytosine and 8-hydroxyguanine were 3.0 and 1.3, respectively. The sensitivity for them were 5.50 x 10(9) mV.s/g and 7.59 x 10(10) mV.s/g, respectively, while their detectable limits were 36.4 pg/s and 15.8 pg/s, respectively. The coefficients of variation for gas chromatograph were less than 5%, for derivatization, less than 6%, and for the whole analysis process, less than 20%.
%K NULL<br>5－甲基胞嘧啶
%K 8－羟基鸟嘌呤
%K GC／FID
%K 分析鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=832139DE54B019A71D8811BFC81A3D48&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=E158A972A605785F&sid=AF507FDD66D991DA&eid=BF112261B65CB9C9&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=4