%0 Journal Article
%T Determination of Chloramphenicol Residues in Milk by Reversed-Phase High Performance Liquid Chromatography with Fluorescence Detection<br>高效液相色谱-荧光检测法测定牛奶中氯霉素的残留量
%A PAN Yingyu
%A XU Qian
%A KANG Xuejun
%A ZHANG Jianxin
%A <br>潘莹宇
%A 许茜
%A 康学军
%A 张建新
%J 色谱
%D 2005
%I 
%X A high performance liquid chromatographic method with fluorescence detection for the determination of chloramphenicol (CAP) residues in milk was developed. Although CAP itself is non-fluorescent the aromatic nitro group of CAP could be reduced to aromatic primary amino group and fluorescamine can be used as a selective reagent for primary amines. Therefore, the CAP was derivatized with fluorescamine prior to injection in this project. The HPLC method was performed on a Diamond C18 column (250 mm x 4.6 mm i. d., 4.0 microm) with the mobile phase composed of sodium acetate buffer (0.02 mol/L, pH 6.0) -acetonitrile-tetrahydrofuran (76: 16: 8, v/v/v). The flow rate of the mobile phase was set at 1.0 mL/min, and the column was maintained at 40 degrees C. Analytes were detected by a fluorescence detector at 410 nm excitation and 508 nm emission wavelength. The standard curve was linear in the range from 0.4 microg/L to 800 microg/L. The limit of detection (LOD) was 0.2 microg/L, and the limit of quantitation (LOQ) was 0.4 microg/L. Overall recoveries were between 66.6% and 92.8% with relative standard deviations between 4.5% and 9.4%. The procedure provides a rapid, reliable and sensitive method for the determination of CAP in milk.
%K high performance liquid chromatography-fluorescence detection
%K pre-column deri-vatization
%K fluorescamine
%K chloramphenicol
%K residues
%K milk<br>高效液相色谱-荧光检测法
%K 柱前衍生化
%K 荧光胺
%K 氯霉素
%K 残留
%K 牛奶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=1E00882B7A633813&yid=2DD7160C83D0ACED&vid=EA389574707BDED3&iid=B31275AF3241DB2D&sid=52B9DFFFCC2EB041&eid=28B3EB92D5061EA4&journal_id=1000-8713&journal_name=色谱&referenced_num=9&reference_num=14