%0 Journal Article
%T Determination of Diosgenin in Rhizoma Paridis by High Performance Liquid Chromatography<br>重楼中薯蓣皂甙元的反相高效液相色谱测定
%A Wei Jianrong
%A Dong Xun
%A <br>韦建荣
%A 董汛
%J 色谱
%D 1999
%I 
%X A method for the separation and determination of diosgenin in Rhizoma Paridis by reversed-phase high performance liquid chromatography was developed. The Rhizoma Paridis powder samples were extracted with methanol in a Soxhlet extractor. After extraction the solvent was evaporated and the extract was hydrolysed with 2 mol/L hydrochloric acid for 2 h in a boiling water bath. Then the diosgenin was extracted with petroleum ether (b.p. 60-90 degrees C). The operating conditions were Symmetry C8 column (5 microns, 3.9 mm x 150 mm) at 30 degrees C, mobile phase of V(acetonitrile):V(water) = 75:25 and UV detector at 203 nm. The linearity of the calibration curve was good in the range of 2.1-10.5 micrograms for diosgenin(r = 0.9994). The average recovery and RSD of diosgenin were 99.1% and 1.7% (n = 5) respectively. The method is accurate and reproducible and has been applied to the analysis of Rhizoma Paridis from different sources.
%K NULL<br>高效液相色谱
%K 薯蓣皂苷元
%K 重楼
%K 测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B8627E61DDD1650F191A&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=94C357A881DFC066&sid=E3C3E274D87A8C16&eid=A03A15CF5604A8B0&journal_id=1000-8713&journal_name=色谱&referenced_num=14&reference_num=5