%0 Journal Article
%T Comparison of the Separation Performances between Semi-Preparative Chromatographic Column and Chromatographic Pie
半制备型柱与饼性能的比较
%A LI Xiang
%A ZHANG Yang-jun
%A GENG Xin-du
%A
李翔
%A 张养军
%A 耿信笃
%J 色谱
%D 2002
%I
%X By comparing the resolution and recoveries of proteins between semi-preparative chromatographic column and chromatographic pie (pie-like column) having the same geometric volume under the same chromatographic conditions, the result from the former was found to have the almost same as that form the latter. However, the back pressure of the chromatographic pie rose slower than that of the chromatographic column with the increase of flow rate. Furthermore, the mass and volume loads for the chromatographic pie were higher than those for the chromatographic column. The results indicated that the chromatographic pie had more advantages than the chromatographic column in their performances, and this lay a foundation of the applications of the chromatographic pie in industry.
%K liquid chromatography
%K semi-preparative chromatographic column
%K semi-preparative chromatographic pie
%K protein renaturation
液相色谱
%K 半制备色谱柱
%K 半制备色谱饼
%K 性能
%K 生物大分子
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=11F7D106E56ABD96&yid=C3ACC247184A22C1&vid=A04140E723CB732E&iid=0B39A22176CE99FB&sid=76B5E24D6EC46B4B&eid=58F693790F887B3B&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=12