%0 Journal Article
%T Preparation and evaluation of an eremomycin-bonded chiral monolithic column for capillary electrochromatography<br>伊瑞霉素键合手性毛细管整体柱的制备与对映体分离
%A LEI Wen
%A ZHANG Lingyi
%A WAN Li
%A ZHU Yaxian
%A QIN Sasa
%A ZHANG Weibing
%A <br>雷雯
%A 张凌怡
%A 万莉
%A 朱亚仙
%A 覃飒飒
%A 张维冰
%J 色谱
%D 2010
%I 
%X Based on the high activity of epoxy group of glycidyl methacrylate monolith, eremomycin-bonded chiral stationary phase (CSP) which has 22 different types of chiral centers was prepared by a one-step derivatization process. The preparation conditions were optimized and the preparation can be carried out in a wide pH range (6.0~9.0). The optimized concentration of eremomycin reactant solution was 42 g/L. The optimized method was simple and mild. Prepared CSP was evaluated by separating racemic mixtures of five amino acids and a chiral drug (rosiglitazone) in capillary electrochromatography mode and all of enantiomers were baseline separated. Analysis time of each pair of enantiomers was less than 4 min, so that rapid analysis can be achieved. The effects of organic modifier, buffer pH, and buffer concentration on the separation were investigated. It shows that eremomycin-bonded CSP has good chiral recognition ability. Chiral recognition mechanisms of different solutes on eremomycin-bonded CSP are discussed.
%K eremomycin
%K macrocyclic antibiotics
%K monolithic chiral stationary phase
%K capillary electrochromatography (CEC)
%K enantiomers<br>伊瑞霉素
%K 大环抗生素
%K 手性整体固定相
%K 毛细管电色谱
%K 对映体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D203736A395BF454672EE66AB680B771&yid=140ECF96957D60B2&vid=D3E34374A0D77D7F&iid=F3090AE9B60B7ED1&sid=1BE4748776BF02C4&eid=88B4027FEBE4F5FF&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=0