%0 Journal Article
%T Determination of simvastatin in human plasma using ultra-performance liquid chromatography- tandem mass pectrometry<br>超高效液相色谱-质谱/质谱联用法测定人血浆中的辛伐他汀
%A WANG Dong
%A QIN Feng
%A CHEN Lingyun
%A HAO Yu
%A ZHANG Yi
%A LI Famei
%A <br>王东
%A 秦峰
%A 陈凌云
%A 郝彧
%A 张轶
%A 李发美
%J 色谱
%D 2008
%I 
%X A rapid, sensitive and specific method has been developed for the determination of simvastatin in human plasma with ultra-performance liquid chromatography-tandem mass spectrometry. Simvastatin and the internal standard (lovastatin) were extracted from human plasma with diethyl ether-n-hexane-isopropanol (80: 20 : 3, v/v/v), then separated on a Waters ACQUITY UPLC BEH C18 column (50 mm x 2.1 mm, 1.7 microm) with isocratic elution at a flow rate of 0.25 mL/min. The mobile phase was composed of 85% acetonitrile and 15% water (containing 10 mmol/L ammonium acetate). Electrospray ionization (ESI) source was applied and operated in positive ion mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 419. 2 --> m/z 199.0 and m/z 405. 0 --> m/z 199.0 was used to quantify simvastatin and the internal standard, respectively. The linear calibration curve was obtained in the concentration range of 0.051 - 20.4 ng/mL. The lower limit of quantification was 0.051 ng/mL. The inter-day and intra-day precision (relative standard deviation) were less than 10%, and the accuracy (relative error) was within -2.7% - 0% calculated from quality control samples. The mean extraction recovery of simvastatin was 91.6%. The method was proved to be selective, sensitive, rapid and suitable for the pharmacokinetic study of simvastatin.
%K 超高效液相色谱-质谱/质谱联用法
%K 辛伐他汀
%K 血药浓度
%K 超高效液相色谱
%K 质谱联用法
%K 测定
%K 人血浆
%K 辛伐他汀
%K tandem
%K mass
%K spectrometry
%K liquid
%K human
%K plasma
%K simvastatin
%K 生物等效性研究
%K 药物动力学
%K 操作
%K 分析速度
%K 灵敏度
%K 方法
%K 平均回收率
%K 相对标准偏差
%K 线性范围
%K 离子检测
%K 多反应监测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=7D5735A40247FFDDD2A7576784402DF0&yid=67289AFF6305E306&vid=96C778EE049EE47D&iid=38B194292C032A66&sid=170CE8B011EA4FD9&eid=6C069F4B248105A5&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=18