%0 Journal Article
%T Isolation and identification of lipopeptides produced by Bacillus subtilis using high performance liquid chromatography and electrospray ionization mass spectrometry<br>高效液相色谱-电喷雾质谱法分离和鉴别枯草芽孢杆菌产生的脂肽类化合物
%A CHEN Hua
%A WANG Li
%A YUAN Chengling
%A ZHENG Zhiming
%A YU Zengliang
%A <br>陈华
%A 王丽
%A 袁成凌
%A 郑之明
%A 余增亮
%J 色谱
%D 2008
%I 
%X Bacillus subtilis JA produces a broad spectrum of bioactive lipopeptides with great potential applications in agriculture and industry. Crude lipopeptides were extracted with 100% methanol from the precipitate which was obtained by adding 6 mol/L HCl to the cell-free culture broth. The crude extract was separated on a Diamonsil C18 column (5 microm, 250 mm x 4.6 mm) in reversed-phase high performance liquid chromatography (HPLC) system to separate the lipopeptide homologues. Five peaks were eluted from HPLC. Electrospray ionization mass spectrometry (ESI-MS) was used to analyze each HPLC fraction. The results showed three series of ion peaks. According to the values of m/z, the three series of ions were classified into surfactin, iturin and fengycin homologues, which were well-known biosurfactants produced by B. subtilis strains. The major ions were structurally characterized using tandem mass spectrometry. This study suggested a reasonable method for the isolation and identification of lipopeptides produced by B. subtilis strains.
%K high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS)
%K Bacillus&prev_q=subtilis')">Bacillus" target="_blank">subtilis')">Bacillus
%K subtilis
%K lipopeptides<br>高效液相色谱-电喷雾质谱法
%K 枯草芽孢杆菌
%K 脂肽类化合物
%K 高效液相色谱
%K 电喷雾
%K 质谱法
%K 分离和鉴别
%K 枯草芽孢杆菌
%K 脂肽类化合物
%K mass
%K spectrometry
%K electrospray
%K ionization
%K liquid
%K chromatography
%K high
%K performance
%K Bacillus
%K subtilis
%K identification
%K 方法
%K 同系物
%K 验证
%K 分子结构
%K 质谱分析
%K 串联
%K 主成分
%K 生物表面活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=8C23985D21D3851C3F8D294977D0F4FA&yid=67289AFF6305E306&vid=96C778EE049EE47D&iid=38B194292C032A66&sid=5957D6E0A50D26B5&eid=15251AE9C02726D3&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=15