%0 Journal Article
%T Rapid Separation and Determination of Enterotoxigenic E.coli by Capillary Zone Electrophoresis<br>ëϸ¹ÜµçÓ¾·¨¿ìËÙ·ÖÀëºÍ¼ì²â³¦¶¾ÐԴ󳦸˾ú
%A CHEN Ping
%A LI Ren kuan
%A XU Xiao hua
%A RAO Ping fan
%A <br>³ÂƼ
%A ÀîÈÊ¿í
%A ÐìС»ª
%A ÈÄƽ·²
%J É«Æ×
%D 2002
%I 
%X Rapid separation and determination of enterotoxigenic E. coli K88, K99 and 987P with intact cells using capillary zone electrophoresis (CZE) are described. The CZE was performed in a running buffer of 0.05 mol/L Na2CO3-NaHCO3 (pH 9.9), under applied voltage of 14.1 kV and detected by an on-line UV at 210 nm. Results show that K88, K99 and 987P were separated completely. Moreover, each strain presented a characteristic peak and a reproducible retention time (RSD < or = 0.9%). The successful analysis of enterotoxigenic E. coli in the excrement of diarrhea piglets is also discussed. K88 and K99 were detected in the excrement of diarrhea piglets born in 5 d - 6 d, and 30 d - 35 d respectively, and nothing was detected in the excrement of diarrhea piglets born in about 60 d.
%K capillary zone electrophoresis
%K separation
%K determination
%K enterotoxigenic
%K E
%K coli
%K excrement of diarrhea piglet<br>ëϸ¹ÜµçÓ¾·¨
%K ·ÖÀë
%K ¼ì²â
%K ³¦¶¾ÐԴ󳦸˾ú
%K ¸¹Ðº
%K ×ÐÖí
%K ·à±ã
%K ·ÖÎö
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=CA379144FE8A2379&yid=C3ACC247184A22C1&vid=A04140E723CB732E&iid=94C357A881DFC066&sid=AC2617B68B137D9D&eid=5319469C819FCFF1&journal_id=1000-8713&journal_name=É«Æ×&referenced_num=4&reference_num=17