%0 Journal Article
%T Determination of Acemetacin and Indometacin in Human Serum by High Performance Liquid Chromatography<br>高效液相色谱法测定人血清中阿西美辛和吲哚美辛
%A HU Yu qin
%A LIU Hui chen
%A MA Rui
%A WANG Jian
%A HOU Yan ning
%A <br>胡玉钦
%A 刘会臣
%A 马锐
%A 王建
%A 侯艳宁
%J 色谱
%D 1999
%I 
%X A method of HPLC for the quantitative determination of acemetacin and indometacin in human serum is described. After being extracted with diethyl ether, acemetacin and indometacin were analyzed by reversed-phase HPLC(Spherisorb-C8) and UV-detector(254 nm), with tolbutamide as internal standard. The mobile phase was a mixture of V(acetate buffer solution, pH 4.6):V(methyl alcohol):V(acetonitrile) = 55:5:40 and at a rate of 1.0 mL/min. Over the mass concentration range of 12.5 micrograms/L-1.6 mg/L, both the calibration curves were linear, r = 0.9996, n = 8. The average recoveries of acemetacin and indometacin were 77.2% and 86.7% respectively. The within-day and between-day RSD of acemetacin and indometacin were less than 5% and 10% respectively.
%K high performance liquid chromatography
%K acemetacin
%K indometacin
%K human serum<br>阿西美辛
%K 吲哚美辛
%K HPLC
%K 人血清
%K 血药浓度
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B8628403BB02B2ED4700&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=B31275AF3241DB2D&sid=CD26609C367AC9C8&eid=D5970ECA7D10A7B1&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=0