%0 Journal Article %T Determination of Acemetacin and Indometacin in Human Serum by High Performance Liquid Chromatography
高效液相色谱法测定人血清中阿西美辛和吲哚美辛 %A HU Yu qin %A LIU Hui chen %A MA Rui %A WANG Jian %A HOU Yan ning %A
胡玉钦 %A 刘会臣 %A 马锐 %A 王建 %A 侯艳宁 %J 色谱 %D 1999 %I %X A method of HPLC for the quantitative determination of acemetacin and indometacin in human serum is described. After being extracted with diethyl ether, acemetacin and indometacin were analyzed by reversed-phase HPLC(Spherisorb-C8) and UV-detector(254 nm), with tolbutamide as internal standard. The mobile phase was a mixture of V(acetate buffer solution, pH 4.6):V(methyl alcohol):V(acetonitrile) = 55:5:40 and at a rate of 1.0 mL/min. Over the mass concentration range of 12.5 micrograms/L-1.6 mg/L, both the calibration curves were linear, r = 0.9996, n = 8. The average recoveries of acemetacin and indometacin were 77.2% and 86.7% respectively. The within-day and between-day RSD of acemetacin and indometacin were less than 5% and 10% respectively. %K high performance liquid chromatography %K acemetacin %K indometacin %K human serum
阿西美辛 %K 吲哚美辛 %K HPLC %K 人血清 %K 血药浓度 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=D7F5F79B7B81B8628403BB02B2ED4700&yid=B914830F5B1D1078&vid=BCA2697F357F2001&iid=B31275AF3241DB2D&sid=CD26609C367AC9C8&eid=D5970ECA7D10A7B1&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=0