%0 Journal Article
%T The Quantitative Method of Pravastatin in Plasma by Gas Chromatograoahy/Mass Spectrometry<br>人体血中美百乐镇（PRAVASTATIN）浓度的气相色谱／质谱测定法
%A Cai Kuihua
%A Tan Bingyan
%A Feng Zhiying
%A Li Zhangwang
%A <br>蔡葵花
%A 谭炳炎
%A 冯智英
%A 李章旺
%A 黄民
%A 赵香兰
%J 色谱
%D 1996
%I 
%X Pravastatin sodium is a cholesterol-lowering agent and potent inhibitor of 3hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase. The plasma concentration is very low. This paper reports a highly sensitive and quantitative method specific for determination of pravastatin in human plasma based on the selective ion monitoring (SIM) mode in gas chromatography/electron impact ionization mass spectrometry. The plasma samples were acidified to pH 4. 0 and then extracted by ethyl acetate. The plasma concentrations of pravastatin in healthy volunteers were detected by selecting m/z 462 as quantitation ion after the sample was methyl esterification with diazomethane and silanization with BSTFA. The minimun detectable limit was 0. 015ng. The standard curve was linear between 1.0 to 60 ng/mL, with a correlation coefficient more than 0. 99. The coefficiens of variation was less than 7.7%. The recovery can reach 90%. This method has been successfully used for the study of pharmacokinetics of pravastatin.
%K gas chromatography/mass spectrum
%K pravastatin
%K plasma concentrations
%K pharmacokinetics<br>气相色谱
%K 质谱
%K 美百乐镇
%K 血药浓度
%K 药代动力学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=473278D7608BFF3ED0DEA6E5560D39CD&yid=8A15F8B0AA0E5323&vid=F3583C8E78166B9E&iid=0B39A22176CE99FB&sid=CDEBD1ACE0A4C1C1&eid=AE09EACBCD1B2A13&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=1