%0 Journal Article
%T Separation of drugs cephalexin, ampicillin, and their biosynthetic precursors L-Phe-L-Cys-D-Val and D-Phe-L-Cys-D-Val by capillary zone electrophoresis]<br>药物头孢Ⅳ号和氨苄青霉素及其生物合成前体的毛细管区带电泳分离
%A Z Wang
%A Y Sun
%A D Wang
%A <br>王志
%A 孙亦梁
%A 王丹丹
%J 色谱
%D 1997
%I 
%X The separation of drugs cephalexin, ampicillin and their biosynthetic precursors L-Phe-L-Cys-D-Val and D-Phe-L-Cys-D-Val was investigated by using capillary zone electrophoresis in acidic Tris-H3PO4 buffer at pH 2.3. With 50 mmol/L Tris-H3PO4 (pH 2.3) buffer, L-Phe-L-Cys-D-Val and D-Phe-L-Cys-D-Val were only partially separated whereas near baseline separation was achieved between cephalexin and ampicillin. The experiments showed that the addition of beta-type cyclodextrins (beta-CD) to the running buffer can influence the separation selectivity. When beta-CD was used as buffer additives at 12 mmol/L, the separation between L-Phe-L-Cys-D-Val and D-Phe-L-Cys-D-Val was much improved, however, the separation between cephalexin and ampicillin was impaired. When using 50 mmol/L Tris-H3PO4 containing 12 mmol/L DM-beta-CD (pH 2.3) buffer system, all compounds investigated were completely separated. This separation method is simple, fast and easy to operate. It involves only the conventional reagents with a much lower run cost than using HPLC. It is expected to be used in the synthetic process monitoring.
%K 毛细管区带电泳
%K 头孢Ⅳ号
%K 氨苄青霉素
%K 前体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6DF69F4D1761D3BDEC&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=94C357A881DFC066&sid=73648F51F187AC5E&eid=A7AE820C12CC9AD3&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=5