%0 Journal Article
%T Determination of melamine residue in plant origin protein powders using high performance liquid chromatography-diode array detection and high performance liquid chromatography-electrospray ionization tandem mass spectrometry<br>高效液相色谱-二极管阵列检测法及高效液相色谱-电喷雾串联质谱法测定植物源性蛋白中残留的三聚氰胺
%A DING Tao
%A XU Jinzhong
%A LI Jianzhong
%A SHEN Chongyu
%A WU Bin
%A CHEN Huilan
%A LI Shujuan
%A <br>丁涛
%A 徐锦忠
%A 李健忠
%A 沈崇钰
%A 吴斌
%A 陈惠兰
%A 李淑娟
%J 色谱
%D 2008
%I 
%X A method for the determination of melamine residue in plant origin protein powders was developed using high performance liquid chromatography-diode array detection (HPLC-DAD) and HPLC-electrospray ionization tandem mass spectrometry (ESI-MS/MS). HPL-DAD was used in preliminary screening of the samples for melamine, and HPLC-MS/MS was used in the confirmatory of melamine. Trichloroacetic acid solution was used to precipitate proteins and to dissociate the target analyte from the sample matrix. The supernatant was cleaned up with strong cation exchange column for HPLC-MS/MS. The HPLC-DAD separation was carried out on a C18 column (250 mm x 4.6 mm, 5 microm) with 0.01 mol/L sodium n-heptanesulfate (pH adjusted to 4.5 with citric acid)-acetonitrile (90:10, v/v) as mobile phase at a flow rate of 1.0 mL/min, and detected at 240 nm. HPLC-MS/MS was performed in selected ion monitoring mode with trichloroacetic acid solution as ion pair reagent. The limits of detection were 10 mg/kg and 0.5 mg/kg for HPLC-DAD and HPLC-MS/MS, respectively. The mean recoveries were 76%-88% for HPLC-DAD and 72%-82% (matrix match calibration curve) for HPLC-MS/MS and the relative standard deviations were 3.4%-6.4% for both HPLC-DAD and HPLC-MS/MS.
%K high performance liquid chromatography-diode array detection (HPLC-DAD)
%K high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
%K melamine residue
%K plant origin protein<br>高效液相色谱-二极管阵列检测
%K 高效液相色谱-电喷雾串联质谱
%K 三聚氰胺残留
%K 植物源性蛋白
%K 高效液相色谱
%K 二极管阵列检测法
%K 电喷雾
%K 串联质谱法
%K 测定
%K 植物
%K 源性
%K 蛋白
%K 残留
%K 三聚氰胺
%K tandem
%K mass
%K spectrometry
%K ionization
%K high
%K performance
%K liquid
%K detection
%K array
%K powders
%K protein
%K origin
%K plant
%K residue
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=7C7E62A36A625991EFA665989F5172E2&yid=67289AFF6305E306&vid=96C778EE049EE47D&iid=CA4FD0336C81A37A&sid=B31275AF3241DB2D&eid=9CF7A0430CBB2DFD&journal_id=1000-8713&journal_name=色谱&referenced_num=62&reference_num=9