%0 Journal Article
%T Simultaneous Determination of Chloramphenicol, Thiam-phenicol, and Florfenicol Residues in Animal Tissues by Gas Chromatography/Mass Spectrometry<br>气相色谱-质谱联用法测定动物组织中氯霉素、氟甲砜霉素和甲砜霉素的残留量
%A LI Peng
%A QIU Yueming
%A CAI Huixia
%A KONG Ying
%A TANG Yingzhang
%A WANG Daning
%A XIE Mengxia
%A <br>李鹏
%A 邱月明
%A 蔡慧霞
%A 孔莹
%A 唐英章
%A 王大宁
%A 谢孟峡
%J 色谱
%D 2006
%I 
%X A method was developed for the simultaneous determination of chloramphenicol (CAP), thiamphenicol (TAP), and florfenicol (FF) residues in animal tissues using gas chromatography/mass spectrometry (GC/MS) with chemical ionization source in negative mode. The homogenized samples were extracted with ethyl acetate and the extracts were partitioned with n-hexane to remove lipids. Further cleanup was performed on a florisil cartridge and the purified samples were derivatized with Sylon BFT N, O-bis (trimethylsilyl) trifluoroacetamide (BSTFA)-trimethylchlorosilane (TMCS), 99: 1, v/v] in toluene. Meta-nitrochloramphenicol (m-CAP) was used as the internal standard for the determination. Selected ion monitoring (SIM) was used for detection, ions were chosen, respectively, for the monitoring at m/z 432, 466, 468, 470 for m-CAP, m/z 376, 378, 466, 468 for CAP, m/z 409, 411, 499, 501 for TAP, and m/z 339, 341, 429, 431 for FF. Quantitative ions were selected at m/z 466 for CAP and m-CAP, at m/z 339 for FF and at m/z 409 for TAP. The detection limits were 0.03 microg/kg for CAP, 0.2 microg/kg for FF and TAP. The correlation coefficients were above 0.99 for the calibration curves of the medicines. The linear ranges were 0.1 - 8.0 microg/kg for CAP and 0.2 - 4.0 microg/kg for FF and TAP. The reproducibilities of the compounds within a batch were 5.5%, 10.4%, and 8.8% for CAP, FF, and TAP respectively, while the corresponding values between the batches were 7.4%, 20.7%, and 19.1%. The recoveries were 80.0% - 111.5%, and the relative standard deviations were 1.2% - 15.4%. The method is applicable for detection of the residues in animal derived food, such as pork, poultry and aquatic products.
%K gas chromatography/mass spectrometry(GC/MS)
%K chloramphenicol
%K florfenicol
%K thiamphenicol
%K solid-phase extraction<br>气相色谱-质谱
%K 氯霉素
%K 氟甲砜霉素
%K 甲砜霉素
%K 固相萃取
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=67BBCE8C9D9B7995&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=CA4FD0336C81A37A&sid=F3583C8E78166B9E&eid=13553B2D12F347E8&journal_id=1000-8713&journal_name=色谱&referenced_num=21&reference_num=13