%0 Journal Article
%T Rapid Analysis of Tryptophan in Serum by High PerformanceLiquid Chromatography with Fluorescence Detection<br>高效液相-荧光检测法快速测定血清中的色氨酸
%A WANG Qing ping
%A TANG Ai guo
%A <br>王清平
%A 唐爱国
%J 色谱
%D 2002
%I 
%X A sensitive, rapid and specific method for the determination of tryptophan(Trp) in serum by high performance liquid chromatography with fluorescence detection (HPLC-FLD) has been established. Serum sample was precipitated by 5% perchloric acid solution and centrifuged to remove protein and then assayed by HPLC-FLD. The operating conditions were Nova-Pak C18 column (3.9 mm i.d x 150 mm, 4 microns), 5 mmol/L KH2PO4 as the mobile phase at a flow rate of 1.0 mL/min. The fluorescence detector was operated at lambda ex 254 nm and lambda em 338 nm. The method was proved to be linear in the range of 0.49 mumol/L-490.00 mumol/L with a regression coefficient of 0.9999. The minimum detection limit was 0.10 mumol/L. The recoveries were 97.2%-98.6%. The intra-batch and inter-batch RSDs were 2.0% and 3.2% respectively. The detection time of serum Trp was within 5 min after injection. The results suggest that this method is simple, fast, accurate and convenient.
%K high performance liquid chromatography
%K fluorescence detection
%K tryptophan
%K serum<br>高效液相色谱法
%K 荧光检测
%K 血清
%K 色氨酸
%K 临床诊断
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=8C459EDD4CFA3FA4&yid=C3ACC247184A22C1&vid=A04140E723CB732E&iid=CA4FD0336C81A37A&sid=286FB2D22CF8D013&eid=E514EE58E0E50ECF&journal_id=1000-8713&journal_name=色谱&referenced_num=5&reference_num=9