%0 Journal Article
%T Simultaneous Determination of Four Compounds in Sanjing Shuang-huanglian Oral Liquid by High Performance Liquid Chroma-<br>高效液相色谱-二极管阵列检测-电化学检测联用技术同时测定三精双黄连口服液中的4种化合物
%A LIU Lin
%A SUO Zhirong
%A ZHENG Jianbin
%A <br>刘琳
%A 秦海燕
%A 郑建斌
%J 色谱
%D 2006
%I 
%X Chlorogenic acid, caffeic acid, baicalin and luteolin in Sanjing Shuanghuanglian Oral Liquid were simultaneously detected and identified using a high performance liquid chromatography coupled with diode array detection and electrochemical detection (HPLC-DAD-ECD). The separation was performed on a Zorbax SB-C18 column (150 mm x 4.6 mm i. d., 5.0 microm). The mobile phase consisted of (A) methanol and (B) methanol-water-acetic acid (50: 50: 1, v/v/v) using a linear gradient elution of 2%A-3%A at 0-3 min, 3%A-25%A at 3-15 min, 25%A-80%A at 15-20 min. The flow rate was 0.8 mL/min. The DAD detection was used at 275 nm. The ECD detection was done at 0.7 V. The column thermostat set at 30 degrees C. The limits of detection of the 4 compounds were 1 mg/L for chlorogenic acid, 0.2 mg/L for caffeic acid, 9 mg/L for baicalin, 7 mg/L for luteolin. The average recoveries were between 96.6%-99.6% with relative standard deviations (RSDs) of 2.5%-4.1%. The method is simple, rapid, reproducible and accurate. It can be used for the routine analysis of the four compounds in Shuanghuanglian Oral Liquid.
%K high performance liquid chromatography(HPLC)
%K diode array detection(DAD)
%K electrochemical detection(ECD)
%K chlorogenic acid
%K caffeic acid
%K baicalin
%K luteolin
%K Sanjing Shuanghuanglian Oral Liquid<br>高效液相色谱法
%K 二极管阵列检测
%K 电化学检测
%K 绿原酸
%K 咖啡酸
%K 黄芩甙
%K 木樨草素
%K 三精双黄连口服液
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=688D5357758F96B2&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=002786F01A86D891&eid=1D01216AD76577EC&journal_id=1000-8713&journal_name=色谱&referenced_num=1&reference_num=9