%0 Journal Article
%T Separation of Sitafloxacin Epimers by Capillary Electrophoresis<br>西他沙星差向异构体的毛细管电泳分离
%A YUAN Pei
%A LIN Lei
%A FAN Qi
%A ZENG Linggao
%A <br>袁佩
%A 林蕾
%A 范琦
%A 曾令高
%J 色谱
%D 2006
%I 
%X Sitafloxacin epimers were separated by capillary zone electrophoresis using gamma-cyclodextrin (gamma-CD) and D-phenylalanine (D-Phe) as chiral selector. The effects of the concentrations of gamma-CD, D-Phe, Cu2+ and pH of buffer were investigated. An uncoated fused-silica capillary of 50 microm i.d. and 60 cm (effective length 52.5 cm) was used. The capillary temperature was maintained at 25 degrees C. Samples were injected under a pressure of 7 kPa for 5 s and separated at 15 kV. A baseline separation of sitafloxacin epimers was achieved with a background electrolyte of 10 mmol/L KH2PO4-K2HPO4(pH 4.5), 10 mmol/L CuSO4, 20 mmol/L gamma-CD and 10 mmol/L D-Phe. The linear range for sitafloxacin was 32 -400 mg/L (0.996). The relative standard deviations (RSDs) of migration time and peak area were less than 1.9% and 3.8% respectively. This method can be applied in qualitative and quantitative analysis for sitafloxacin epimers.
%K capillary electrophoresis(CE)
%K sitafloxacin
%K epimers<br>毛细管电泳
%K 西他沙星
%K 差向异构体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A6720FCB5179B61E&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=F9A6B6F259CE5121&eid=F204392B3B11C3BD&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=7