%0 Journal Article
%T Purification of Superoxide Dismutase from Bovine Blood by Medium Pressure Liquid Chromatography(MPLC)<br>中压液相色谱纯化牛血红细胞超氧化物歧化酶
%A Han Qinghong
%A Wang Zhenshan
%A Xu xiaoying
%A Sun Qinmei
%A Chen Fulai & Zhang Naizhong
%A <br>韩庆宏
%A 汪振珊
%A 徐晓莹
%A 孙琴美
%A 陈福来
%A 张乃忠
%J 色谱
%D 1994
%I 
%X A rapid and effective purification of superoxide dismutase from bovine erythrocytes by MPLC is repoted.After bovine erythrocytes were treated with chloroform-ethanol to remove hemoglobin,acetone was used toremove contaminant proteins.Then highly purified Cu-Zn SOD(the specific activity was 10 000～15 000U/mg pr.)was obtained by twice DEAE-Sepharose chromatography using different eluting procedures.SDS-PAGE of the Cu-Zn SOD purified as above revealed one band. The molecular weight was 1 5 000～17 000.
%K medium pressure liquid chromatography
%K superoxide dismutase
%K erythrocyte<br>牛
%K 红细胞
%K 超氧物歧化酶
%K 色谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=8B58229E786C0319A287EF10BDD58C26&yid=3EBE383EEA0A6494&vid=59906B3B2830C2C5&iid=CA4FD0336C81A37A&sid=5D71B28100102720&eid=CB423C9A71560A74&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=7