%0 Journal Article
%T Determination of Steroids in Milk Powder and Birds'' Feces by High Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detector<br>高效液相色谱/挥发激光散射检测器测定奶粉和鸟粪中的甾体化合物
%A Feng Aisheng
%A Zou Hanfa
%A Zhang Bing
%A Wang Hailin
%A Zhang Yukui
%A <br>冯埃生
%A 邹汉法
%A 张冰
%A 汪海林
%A 张玉奎
%J 色谱
%D 1997
%I 
%X In this paper, a new, simple and rapid method for the analysis of steroids by reversed-phase high performance liquid chromatography (HPLC) coupled with evaporative light scattering detector (ELSD) is presented. A good resolution of the 12 steroids is obtained within 35 min with a gradient elution using a binary mobile phase composed of methanol and water. The analytical column used was 2.0mm x 150mm, packed with BDS (particle size 5microm) and the mobile phase flow rate was 0.2mL/min. Nebulizer gas flow rate and drift tube temperature, the two critical parameters for determining the reproducibility and signal-to-noise ratio of ELSD were 2.00mL/min and 95 degrees C, respectively. The response of the ELSD was non-linear as a result of basic light-scattering principles, the calibration curve by plotting lnY vs lnX (Y, X stand for peak area and concentration, respectively) shows a good linearity in the range of 5-50mg/L with a correlation coefficient higher than 0.99 for individual steroid and with relative standard deviation less than 6.92%. This method has been applied to the determination of steroids in milk powder and birds' feces, solid phase extraction on reverse phase bonded C18 (5mL/500mg) was used to treat the samples efficiently. The recoveries of the steroids were between 84.0%-102.8%.
%K reversed phase high performance liquid chromatography
%K evaporative light scattering detector
%K milk
%K feces
%K steroids<br>高效液相色谱法
%K 奶粉
%K 鸟粪
%K 甾体化合物
%K ELSD
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6D53A5759FCC13C2C7&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=0B39A22176CE99FB&sid=331211A5F5616413&eid=03F1579EF92A5A32&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=3