%0 Journal Article
%T Research on the Reproducibility and Selection of Experimental Conditions of Capillary Electrochromatography<br>毛细管电色谱柱重复性考察及实验条件的选择
%A Shi Wei
%A Zhang Lihua
%A Dong Lifu
%A Zou Hanfa
%A Zhang Yukui
%A <br>施维
%A 张丽华
%A 董礼孚
%A 邹汉法
%A 张玉奎
%J 色谱
%D 1997
%I 
%X In this work, we prepared the packed columns for capillary electrochromatography (CEC) with 75microm and 100microm i.d., both of coupled mode and uncoupled mode. A mixture of thiourea and other three aromatic compounds was employed to evaluate the efficiency of the CEC columns. RSD values of retention times smaller than 2.12% was obtained on the home-made CEC system. The influence of pH value and the concentration of the organic solvent in the buffer on the retention behavior was investigated, giving a guide to the selection of experimental conditions. High speed analysis can be achieved by using high pH value buffers. Higher concentration of acetonitrile will improve the elution ability, while lower ocncentration of acetonitrile would improve the resolution. Seven kinds of neutral molecules were separated efficiently within 25 minutes. The results from the two modes of CEC columns were compared.
%K capillary electrochromatography
%K capillary packed column
%K reproducibility
%K experimental conditions<br>毛细管电色谱法
%K 毛细管填充柱
%K 色谱柱
%K CEC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6D6428F981A644E7AB&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=38B194292C032A66&sid=BC084ACE66B62CC8&eid=38685BC770C663F2&journal_id=1000-8713&journal_name=色谱&referenced_num=4&reference_num=2