%0 Journal Article
%T Determination of Phospholipid in the Peroxisomal Membrane of Rat Liver by High Performance Liquid Chromatography<br>高效液相色谱法测定大鼠肝过氧化物酶体膜磷脂
%A JIANG Qiu-fen
%A LUO Zi-sheng
%A LI Xing-fa
%A JIANG Ling-ling
%A <br>姜秋芬
%A 骆子生
%A 李兴发
%A 姜玲玲
%J 色谱
%D 2000
%I 
%X A method was employed for the determination of phospholipid in membrane of peroxisomes by high performance liquid chromatography. The separation was performed on a mu-Porasil Si60 column(250 mm x 4.6 mm i.d.) by gradient elution. The phospholipids were detected at 205 nm. The limits of detection for cardiolipin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin were 12, 8, 23, 4, 23 and 7 ng respectively and the recoveries were between 84% and 103%. The linear relationship of calibration curves was good (r > 0.91). The phospholipids in peroxisomal membrane of 10 SD rat liver were determined and the results are satisfactory.
%K high performance liquid chromatography
%K peroxisomal membrane
%K phospholipid
%K rat<br>高效液相色谱法
%K 大鼠肝
%K 过氧化物酶体膜
%K 磷脂
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=9BA2AC08419A7FD82787DF13837262CC&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=0B39A22176CE99FB&sid=E22B6B8FE86DD8F9&eid=3986B25773CB6C30&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=4