%0 Journal Article
%T Determination of Docosyl Ferulate in Extract of Pygeum Africanum Hook. by High Performance Liquid Chromatography(HPLC)<br>高效液相色谱法测定非洲刺李提取物中阿魏酸二十二烷酯
%A Y You
%A G Cheng
%A <br>游勇基
%A 程广强
%J 色谱
%D 1997
%I 
%X This paper reports a method for the determination of docosyl ferulate in the extract of bark of pygeum africanum Hook. by HPLC. After the sample was pretreated, the docosyl ferulate was well separated and determined on a Spherisorb C18 column (250 x 4.6mm, 5microm) using a mobile phase of methanol with a flow rate of 1mL/min. The column temperature was selected at 40 degrees C to avoid tailing of peak. UV detection was performed at 326nm. In order to confirm the docosyl ferulate separated from sample, the peak apex at 10.4 minute was scanned from 195nm to 360nm by photodiode array detector. Its spectrum showed the maxium absorption peak at 240nm and 326nm corresponding with the spectrum of docosyl ferulate. The linear correlation was observed from the 10mg/L to 100mg/L of docosyl ferulate (r = 0.9995). The average recovery was 98.4% +/- 1.98%. Three batches of sample were determined.
%K NULL<br>高效液相色谱
%K 阿魏酸
%K 二十二烷酯
%K 非洲刺李
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=A0C7CB2C65AD7C6DF935966D5AB5D43D&yid=5370399DC954B911&vid=23CCDDCD68FFCC2F&iid=38B194292C032A66&sid=0F7768518993EDDE&eid=89AC6B0ADBEA2741&journal_id=1000-8713&journal_name=色谱&referenced_num=2&reference_num=5