%0 Journal Article
%T Determination of Ferulic Acid in Chuanxiong and in Animal Serum and Cerebrospinal Fluid by Reversed-Phase High Performance Liquid Chromatography<br>反相高效液相色谱法测定川芎饮片、实验大鼠血清和脑匀浆中的阿魏酸
%A L
%A <br>吕琨
%A 丁明玉
%A 李红霞
%A 刘德麟
%J 色谱
%D 2000
%I 
%X An easy, rapid and sensitive method for the determination of ferulic acid(FA) in Chuanxiong extracts, animal (mouse) serum and cerebrospinal fluid by RP-HPLC has been developed. The FA was separated on an ODS column, Nova-Pak C18(3.9 mm i.d. x 150 mm) and detected at the wavelength of 320 nm. The mobile phase was methanol-water-acetic acid (35:65:0.5, V/V), with a flow rate of 0.8 mL/min. The detection limit of FA was 1.7 micrograms/L(S/N = 3) and the calibration curve was linear within the range of 0.85 mg/L-4.00 mg/L(r = 0.99904, n = 6). The mean recovery from animal serum and cerebrospinal was 95%-102%.
%K reversed
%K phase high performance liquid chromatography
%K ferulic acid
%K Chuanxiong(
%K Ligusticum wallichii Franch
%K )
%K serum
%K cerebrospinal fluid<br>反相高效液相色谱法
%K 阿魏酸
%K 川芎
%K 血清
%K 脑匀浆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=3897F354C585FD7ABF2BA3D6899E1454&yid=9806D0D4EAA9BED3&vid=13553B2D12F347E8&iid=B31275AF3241DB2D&sid=AE43DE0664B02889&eid=F3A3627BFEF439C1&journal_id=1000-8713&journal_name=色谱&referenced_num=5&reference_num=9