%0 Journal Article
%T Three-Step Column Chromatographic Method for Separation and Purification of Thrombin-Like Enzyme from Venom of Agkistrodon halys pallas<br>三步柱色谱法从江浙蝮蛇蛇毒中分离纯化类凝血酶
%A BIAN Liujiao
%A YANG Xiaoyan
%A LIU Li
%A <br>边六交
%A 杨晓燕
%A 刘莉
%J 色谱
%D 2006
%I 
%X A three-step column chromatographic method, utilizing CM Sepharose CL-6B, DEAE Sepharose Fast Flow and Sephadex G-75 in sequence, for the separation and purification of thrombin-like enzyme from crude venom of Agkistrodon halys pallas was developed. Based on the separation results on lab-scale chromatographic column, this isolation and purification process was amplified according to the diameter of the chromatographic columns used in lab-scale. It was found that when the amount of the sample loaded on the large-scale columns was 25 times as that loaded on the lab-scale columns, the quality level of the thrombin-like enzyme obtained with the large-scale columns was almost the same as that obtained with lab-scale columns. The relative molecular mass of thrombin-like enzyme obtained was about 33 500, and its purity was about 96%. When using this method to isolate thrombin-like enzyme from crude venom of Agkistrodon halys pallas, the total protein yield and total activity yield of thrombin-like enzyme were about 0.3% and 64% respectively, and specific activity was more than 2 000 U/mg.
%K three-step column chromatographic method
%K thrombin-like enzyme
%K snake venom
%K Agkistrodon halys pallas
%K separation and purification<br>三步柱色谱
%K 类凝血酶
%K 蛇毒
%K 江浙蝮蛇
%K 分离纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=1AE901E1A1AC27E7&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=5E25104E99903E8A&eid=12DC19455C3A2FA8&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=20