%0 Journal Article
%T Determination of Puerarin in Kangshen Granule by Reversed-Phase High Performance Liquid Chromatography<br>反相高效液相色谱法测定康肾颗粒中的葛根素
%A CHEN Man
%A SHI Jian
%A WANG Lili
%A GENG Xindu
%A <br>陈曼
%A 史键
%A 王骊丽
%A 耿信笃
%J 色谱
%D 2006
%I 
%X Kangshen granule, containing 12 kinds of traditional Chinese medicine, has complex composition. A new method for the determination of puerarin in Kangshen granule was developed using reversed-phase high performance liquid chromatography coupled with matrix assisted laser desorption ionization time of flight mass spectrometry and ultraviolet detection (RP-HPLC/MALDI-TOF MS/UV). The sample was separated by RP-HPLC with a non-linear gradient elution, and then was qualitatively detected by mass spectrometry to identify the puerarin peak. The chromatographic process was performed on a YMC-ODS column under the optimized conditions with a mobile phase consisting of methanol, water and 0.1% TFA, and detected at 250 nm. The linear range of puerarin was from 0. 132 to 1.32 microg (r = 0.9997). The recovery was higher than 103% and the relative standard deviation was lower than 2.0% (n=6). The puerarin content in Kangshen granule sample was determined and the puerarin content was 3.09 mg/g. The method is simple and feasible for the determination of puerarin with a good repeatability. The method can be used for quality control of Kangshen granule.
%K reversed-phase high performance liquid chromatography(RP-HPLC)
%K matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS)
%K ultraviolet detection
%K puerarin
%K Kangshen granule<br>反相高效液相色谱法
%K 基质辅助激光解吸离子化飞行时间质谱
%K 紫外检测
%K 葛根素
%K 康肾颗粒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=6DD2F63ADB3050BC&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=F176272DD933A0BB&eid=ABF2590617D31FFD&journal_id=1000-8713&journal_name=色谱&referenced_num=5&reference_num=10