%0 Journal Article
%T Simultaneous Determination of Five Isoflavonoids in Commercial Radix Astragali by High Performance Liquid Chromatography<br>高效液相色谱法同时测定黄芪中的五种异黄酮类成分
%A WANG Xiaohui
%A LIU Tao
%A LI Qing
%A CHEN Xiaohui
%A BI Kaishun
%A <br>王晓辉
%A 刘涛
%A 李清
%A 陈晓辉
%A 毕开顺
%J 色谱
%D 2006
%I 
%X A reversed-phase high performance liquid chromatographic method (RP-HPLC) was developed for the simultaneous analysis of five isoflavonoids of Huangqi, the roots of Astragalus mongholicus. HPLC determination was performed on a Diamonsil C18 column (200 mm x 4.6 mm i.d., 5 microm) and detected at 230 nm. The mobile phase consisted of (A) acetonitrile and (B) water. Gradient program was adopted as follows: 0-25 min from 17% A to 31% A, 25 -45 min 35% A. The flow rate was maintained at 1.0 mL/min. The method was proved to be linear in the ranges of 20.12-201.2 mg/L (r = 0.9992), 4.62-46.2 mg/L (r = 0.9997), 4.86 -48.6 mg/L (r = 0.9997), 9.24-92.4 mg/L (r = 0.9995) and 6.92-69.2 mg/L (r = 0. 999 5) for the five isoflavonoids, calycosin-7-O-beta-D-glucoside, formononetin-7-O-beta-D-glucoside,9,10-dimethoxypterocarpan-3-O-beta-D-glucoside, calycosin and formononetin, respectively. The recoveries were greater than 94%, and relative standard deviations were less than 3.2%. The method has been successfully applied to the simultaneous determination of the five isoflavonoids of radix astragali.
%K reversed-phase high performance liquid chromatography(RP-HPLC)
%K isoflavonoid
%K radix astragali<br>反相高效液相色谱法
%K 异黄酮
%K 黄芪
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=B3541289DD7A8EFB&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=8C8D895E58E44DBB&eid=8CE1095CD639AEF4&journal_id=1000-8713&journal_name=色谱&referenced_num=7&reference_num=6