%0 Journal Article
%T Separation of Enantiomers of Amino Acid Derivatives by High Performance Liquid Chromatography on Teicoplanin Chiral Stationary Phase<br>大环糖肽抗生素键合相高效液相色谱法拆分7种氨基带保护基的氨基酸对映体
%A LIN Lin
%A XIA Lijun
%A XU Xu
%A XU Hongyan
%A <br>林琳
%A 夏立钧
%A 许旭
%A 徐红岩
%J 色谱
%D 2006
%I 
%X Direct enantioseparations of seven N-fluorenylmethoxycarbonyl-amino acids (Fmoc-Val, Fmoc-Ser, Fmoc-Leu, Fmoc-Ile, Fmoc-Met, Fmoc-Ala, and Fmoc-Phe) were achieved by high performance liquid chromatography using a 250 mm x 4. 6 mm i.d. column bonded teicoplanin on silica gel as chiral stationary phase and methanol-acetic acid-triethylamine mixtures as mobile phase. The comparison of retention behavior and enantioseparation of N-Fmoc-Val and its non-blocked analogue on a Chirobiotic column with different mobile phase systems is presented. The effects of the content of acetic acid and triethylamine and the content ratio of acetic acid and triethylamine in the mobile phase on the enantioseparation of some N-Fmoc-amino acids were examined. The results showed that variations of column temperatureand flow rate of mobile phase also had effects on enantioseparation of N-Fmoc-amino acids. The method had been successfully applied to the determination of optical purity of some derivatized amino acids.
%K high performance liquid chromatography
%K enantioseparation
%K teicoplanin chiral stationary phase
%K N-fluorenylmethoxycarbonyl-amino acid enantiomers<br>高效液相色谱法
%K 对映体拆分
%K 大环糖肽抗生素手性固定相
%K 氨基带芴甲氧羰基保护基的氨基酸对映体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=252B886AB89423A0&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=3986B25773CB6C30&eid=2922B27A3177030F&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=13