%0 Journal Article
%T Detection of Acetylbritannilactone in Inulicin Ingredients and Dynamic Change in Vivo by Micellar Electrokinetic Capillary Chromatography<br>在线推扫富集-胶束电动毛细管色谱检测旋覆花素中和大鼠血浆中的旋覆花内酯
%A ZHAO Jingshan
%A WEN Jinkun
%A HAN Mei
%A <br>赵京山
%A 温进坤
%A 韩梅
%J 色谱
%D 2006
%I 
%X A method was established for the determination of acetylbritannilactone (ABL) in inulicins and rat plasma by micellar electrokinetic capillary chromatography (MECC), in which on-line sweeping technique was applied to elevate the sensitivity of the detection. The column used was an uncoated fused silica capillary with a size of 57 cm (effective length of 50 cm) x 75 microm i.d. Electrode buffer was a 50 mmol/L borate buffer containing 50 mmol/L sodium lauryl sulfate (SDS) (pH 9.5). Sample buffer was a 10 mmol/L borate buffer (pH 9.5). Other conditions were as follows: separation voltage, 23 kV; detection wavelength, 195 nm; sample injection, 3.45 kPa (0.5 psi) x 5 s; column temperature, 25 degrees C. The on-line sweeping-MECC had excellent linearity with correlation coefficient of 0. 997 5, and reproducibility with the relative standard deviation of ABL migration time less than 5%. The recovery was more than 92%, and the sensitivity was 0.005 g/L. ABL in inulicin and their dynamic change in vivo were determined by sweeping-MECC. The results indicate that on-line sweeping-MECC is a simple and rapid method for the determination of trace biological sample. The method needs only micro-amount of samples, and is characterized by higher degree of automation and sensitivity.
%K micellar electrokinetic capillary chromatography(MECC)
%K on-line sweeping technique
%K acetylbritannilactone
%K inulicin
%K rat plasma<br>胶束电动毛细管色谱
%K sweeping
%K 在线富集技术
%K 旋覆花内酯
%K 旋覆花素
%K 大鼠血浆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=BD6074E203A43D92&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=37F781FD8E744761&eid=D8AE57480552698F&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=14