%0 Journal Article
%T Sample Pre-Processing for Mass Spectrometric Analysis of Glycoprotein Structure<br>糖蛋白结构质谱解析的样品前处理
%A ZHOU Wei
%A LIU Xiaohui
%A ZHOU Xinwen
%A SHEN Huali
%A YANG Pengyuan
%A <br>周玮
%A 刘晓慧
%A 周新文
%A 申华莉
%A 杨芃原
%J 色谱
%D 2007
%I 
%X A novel,simple and fast method based on a quadrupole ion trap(QIT) and a time-of-flight mass spectrograph was developed for the glycosylation site and glycan structure analysis of glycoprotein by in-solution digestion and in-gel digestion which did not need to enrich and label the glycopeptide.Horseradish peroxidase(HRP) and RNase B were utilised to optimize the conditions of sample pre-processing.The effects of the conditions were investigated separately and the optimal conditions were:heat denaturation of RNase B,chemical denaturation of HRP,RNase B digestion with endoproteinase Lys-C,HRP digestion with trypsin,12-16 h of digestion,50% acetonitrile-5% trifluoroacetic acid of the extraction solution,sandwich spotting method of the sample.
%K glycoprotein structure
%K mass spectrometric analysis
%K sample pre-preparation
%K conditions of optimization<br>糖蛋白结构
%K 质谱解析
%K 样品前处理
%K 条件优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=21B7391286A2A2A2&yid=A732AF04DDA03BB3&vid=C5154311167311FE&iid=94C357A881DFC066&sid=F8AEC975DBDD7F2F&eid=821800203AD09E7B&journal_id=1000-8713&journal_name=色谱&referenced_num=0&reference_num=19