%0 Journal Article
%T Extraction of Three Tanshinones from the Root of Salvia miltiorrhizaBunge by Supercritical Carbon Dioxide Fluid and Their Analysis with High Performance Liquid Chromatography<br>丹参中3种丹参酮的超临界二氧化碳萃取及液相分析
%A LI Ying chun
%A ZENG Jian qing
%A Liu Li mei
%A JIN Xue song
%A <br>李迎春
%A 曾健青
%A 刘莉玫
%A 金雪松
%J 色谱
%D 2002
%I 
%X The extraction of three tanshinones from the root of Salvia miltiorrhiza Bunge by supercritical carbon dioxide (SC-CO2) fluid with ethanol as co-solvent was investigated using four-factor and three-level orthogonal design method and the systematic method. The optimized conditions were established as follows: 95% ethanol was selected as a co-solvent, flow rate of co-solvent was 1.0 mL/min, extraction pressure was 20 MPa, extraction temperature was 45 degrees C, separation temperature was 35 degrees C. The sample solution was separated on a Nova-Pak C18 column (3.9 mm i.d. x 300 mm, 5 microns) with CH3OH-H2O (80:20, V/V) as the mobile phase and detected at 280 nm. The correlation coefficients were 0.9994-0.9998 and the relative standard deviations were 2.37%-3.47%. The detection limits were 0.473 mg/L, 0.184 mg/L and 0.452 mg/L for tanshinone-II A, tanshinone-I and cryptotanshinone respectively.
%K NULL<br>丹参酮ⅡA
%K 丹参酮Ⅰ
%K 超临界二氧化碳
%K 萃取
%K 高效液相色谱法
%K 丹参
%K 隐丹叁酮
%K 中药分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=4D81E042D77AFEC6881D14759692069C&aid=15E9AA06FB6FC4D0&yid=C3ACC247184A22C1&vid=A04140E723CB732E&iid=CA4FD0336C81A37A&sid=1371F55DA51B6E64&eid=ECE8E54D6034F642&journal_id=1000-8713&journal_name=色谱&referenced_num=21&reference_num=4